Quantifi cation of growth promoters olaquindox and carbadox in animal feedstuff with the Agilent 1260 Infi nity Binary LC system with UV detection

Simple, sensitive analytical methods are required for routine monitoring of banned substances, such as antibiotics that are added to animal feed to prevent disease and promote growth. This Application Note demonstrates the quantifi cation of two growth promoters, olaquindox and carbadox in animal feedstuff using the Agilent 1260 Infi nity Binary LC system. The 10x higher sensitivity of the 60 mm path length Max-Light fl ow cell enabled the detection of low levels of the two analytes present in animal feed. The detection and quantifi cation of olaquindox and carbadox at wavelengths showing higher absorption, 375 nm and 306 nm respectively, improved the detection limits of the two analytes. The lower limit of quantifi cation achieved for both molecules was 50 ng/mL. The area response was found to be linear up to 5,000 ng/mL. A chicken feed sample, prepared by a simple extraction procedure using 1:1 methanol/water yielded recoveries > 80% for olaquindox and carbadox. Author Srividya Kailasam Agilent Technologies, Inc. Bangalore, India